Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells

PLoS One. 2015 Jun 22;10(6):e0130685. doi: 10.1371/journal.pone.0130685. eCollection 2015.

Abstract

In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3'-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects
  • Cell Cycle / drug effects
  • Cell Line, Tumor
  • Cell Movement / drug effects*
  • Cell Proliferation / drug effects*
  • Female
  • Flavonoids / pharmacology*
  • Humans
  • Luteolin / pharmacology
  • Matrix Metalloproteinase 2 / metabolism
  • Matrix Metalloproteinase 9 / metabolism
  • Ovarian Neoplasms / metabolism
  • Ovarian Neoplasms / pathology*
  • Potamogetonaceae / chemistry*
  • RNA, Messenger / drug effects

Substances

  • Flavonoids
  • RNA, Messenger
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 9
  • Luteolin

Grants and funding

This study was financially supported by National Natural Science Foundation of China (No. 31200426), National Water Special Project (No. 2012ZX07203-004), Science and Technology Planning Project of Shandong Province (No. 2011GGH21605), and Natural Science Foundation of Shandong Province of China (No.ZR2014YL001). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.