Treponema DNA in bovine 'non-healing' versus common sole ulcers and white line disease

Sabine Sykora; Johann Kofler; Johanna Glonegger-Reichert; Johanna Dietrich; Gloria Auersperg; Sabine Brandt

doi:10.1016/j.tvjl.2015.05.023

Treponema DNA in bovine 'non-healing' versus common sole ulcers and white line disease

Vet J. 2015 Sep;205(3):417-20. doi: 10.1016/j.tvjl.2015.05.023. Epub 2015 Jun 1.

Authors

Sabine Sykora¹, Johann Kofler², Johanna Glonegger-Reichert², Johanna Dietrich², Gloria Auersperg¹, Sabine Brandt³

Affiliations

¹ Research Group Oncology, Equine Clinic, Department of Companion Animals and Horses, University of Veterinary Medicine, Veterinaerplatz 1, Vienna A-1210, Austria.
² Clinic for Ruminants, Department of Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinaerplatz 1, Vienna A-1210, Austria.
³ Research Group Oncology, Equine Clinic, Department of Companion Animals and Horses, University of Veterinary Medicine, Veterinaerplatz 1, Vienna A-1210, Austria. Electronic address: sabine.brandt@vetmeduni.ac.at.

PMID: 26093913
DOI: 10.1016/j.tvjl.2015.05.023

Abstract

Bovine digital dermatitis (BDD)-associated 'non-healing' white line disease (nhWLD) and 'non-healing' sole ulcers (nhSU) are increasingly encountered in cattle. Using established PCR protocols, 42 nhWLD/nhSU, 25 BDD and 15 common WLD DNA isolates were screened for the presence of Treponema DNA. Obtained amplicons were identified by gel electrophoresis and sequencing. Independent from their source, Treponema DNA was isolated from all lesions, but the lesion type varied with the detected Treponema phylotypes. Whilst Treponema pedis was omnipresent, T. medium was almost exclusively identified in BDD and associated nhWLD/nhSU lesions when compared to common WLD. This observation was confirmed by specific T. medium PCR scoring positive for all BDD and nhWLD/nhSU lesions, but for only 1/15 (6.7%) common WLD lesions. It is suggested that T. medium may have an active role in the pathogenesis of nhWLD/nhSU but further work is needed to verify this concept.

Keywords: Bovine digital dermatitis; Cattle; Non-healing sole ulcers; Non-healing white line disease; Treponema.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cattle Diseases / microbiology*
DNA, Bacterial
Dairying
Digital Dermatitis / microbiology*
Female
Treponema / classification
Treponema / genetics*
Ulcer / microbiology
Ulcer / physiopathology
Ulcer / veterinary*
Wound Healing

Substances

DNA, Bacterial