A doubly responsive probe for the detection of Cys4-tagged proteins

Chem Commun (Camb). 2015 Jul 21;51(57):11482-4. doi: 10.1039/c5cc04721h.

Abstract

Recombinant proteins bearing a tag are crucial tools for assessing protein location or function. Small tags such as Cys4 tag (tetracysteine; Cys-Cys-X-X-Cys-Cys) are less likely disrupt protein function in the living cell than green fluorescent protein. Herein we report the first example of the design and synthesis of a dual fluorescence and hyperpolarized (129)Xe NMR-based sensor of Cys4-tagged proteins. This sensor becomes fluorescent when bound to such Cys4-tagged peptides, and the (129)Xe NMR spectrum exhibits a specific signal, characteristic of the biosensor-peptide association.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Biosensing Techniques
  • Cysteine / analysis*
  • Fluoresceins / chemistry*
  • Fluorescent Dyes / chemistry*
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • Organometallic Compounds / chemistry*
  • Peptides / analysis*
  • Polycyclic Compounds / chemistry*
  • Recombinant Proteins / analysis
  • Spectrometry, Fluorescence
  • Xenon Isotopes / chemistry

Substances

  • CrAsH compound
  • Fluoresceins
  • Fluorescent Dyes
  • Organometallic Compounds
  • Peptides
  • Polycyclic Compounds
  • Recombinant Proteins
  • Xenon Isotopes
  • cryptophane
  • 6-carboxyfluorescein
  • Cysteine