Abstract
We propose a novel enzyme-labeling method for DNA aptamers using enzyme-fused zinc finger proteins. We achieved thrombin detection and vascular endothelial growth factor detection using zinc finger-fused firefly luciferase.
MeSH terms
-
Animals
-
Aptamers, Nucleotide / chemistry*
-
Biosensing Techniques
-
Cattle
-
Enzyme-Linked Immunosorbent Assay
-
Humans
-
Luciferases, Firefly / chemistry*
-
Luminescent Agents / chemistry*
-
Luminescent Measurements
-
Thrombin / analysis*
-
Vascular Endothelial Growth Factor A / analysis*
-
Zinc Fingers
Substances
-
Aptamers, Nucleotide
-
Luminescent Agents
-
Vascular Endothelial Growth Factor A
-
Luciferases, Firefly
-
Thrombin