Enzyme linking to DNA aptamers via a zinc finger as a bridge

Chem Commun (Camb). 2015 Jul 21;51(57):11467-9. doi: 10.1039/c5cc02906f.

Abstract

We propose a novel enzyme-labeling method for DNA aptamers using enzyme-fused zinc finger proteins. We achieved thrombin detection and vascular endothelial growth factor detection using zinc finger-fused firefly luciferase.

MeSH terms

  • Animals
  • Aptamers, Nucleotide / chemistry*
  • Biosensing Techniques
  • Cattle
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Luciferases, Firefly / chemistry*
  • Luminescent Agents / chemistry*
  • Luminescent Measurements
  • Thrombin / analysis*
  • Vascular Endothelial Growth Factor A / analysis*
  • Zinc Fingers

Substances

  • Aptamers, Nucleotide
  • Luminescent Agents
  • Vascular Endothelial Growth Factor A
  • Luciferases, Firefly
  • Thrombin