Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients

O E Bryzgunova; S N Tamkovich; A V Cherepanova; S V Yarmoshchuk; V I Permyakova; O Y Anykeeva; P P Laktionov

Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients

Acta Naturae. 2015 Apr-Jun;7(2):115-8.

Authors

O E Bryzgunova¹, S N Tamkovich², A V Cherepanova¹, S V Yarmoshchuk³, V I Permyakova¹, O Y Anykeeva³, P P Laktionov⁴

Affiliations

¹ Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prosp. Lavrentieva, 8, 630090, Novosibirsk, Russia.
² Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prosp. Lavrentieva, 8, 630090, Novosibirsk, Russia ; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.
³ Meshalkin Novosibirsk State Research Institute of Circulation Pathology, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia.
⁴ Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prosp. Lavrentieva, 8, 630090, Novosibirsk, Russia ; Meshalkin Novosibirsk State Research Institute of Circulation Pathology, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia.

PMID: 26085953
PMCID: PMC4463421

Abstract

A direct correlation between the concentration of cell-free and cell-surface-bound circulating DNA (cfDNA and csbDNA, respectively) was demonstrated. Based on an inverse correlation between blood plasma DNase activity and the cfDNA concentration, blood DNases are supposed to regulate the cfDNA concentration. However, no correlation was found between the DNase activity in blood plasma and the csbDNA concentration, indicating that blood DNases are not involved in csbDNA dissociation from the cell surface. The possibility of DNA redistribution between cfDNA and csbDNA indicates that the total pool of circulating DNA (cfDNA + csbDNA) should be used for a correct analysis of marker DNA concentrations and data standardization.

Keywords: DNase activity; circulating DNA; plasma; prostate cancer.