Structure of the O-specific polysaccharide from the legume endosymbiotic bacterium Ochrobactrum cytisi strain ESC1(T)

Malgorzata Pac; Iwona Komaniecka; Katarzyna Zamlynska; Anna Turska-Szewczuk; Adam Choma

doi:10.1016/j.carres.2015.05.002

Structure of the O-specific polysaccharide from the legume endosymbiotic bacterium Ochrobactrum cytisi strain ESC1(T)

Carbohydr Res. 2015 Sep 2:413:37-40. doi: 10.1016/j.carres.2015.05.002. Epub 2015 May 18.

Authors

Malgorzata Pac¹, Iwona Komaniecka¹, Katarzyna Zamlynska¹, Anna Turska-Szewczuk¹, Adam Choma²

Affiliations

¹ Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
² Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland. Electronic address: adam.choma@poczta.umcs.lublin.pl.

PMID: 26083199
DOI: 10.1016/j.carres.2015.05.002

Abstract

The O-specific polysaccharide was obtained from the lipopolysaccharide of the legume-endosymbiotic bacterium Ochrobactrum cytisi strain ESC1(T) and studied by chemical analyses and 1D and 2D NMR spectroscopy. The polysaccharide was found to have a disaccharide repeating unit containing α-d-fucose and β-N-acetyl-d-galactosamine residues connected via (1→3)-glycosidic bonds, resulting in the following structure: →3)-α-d-Fucp-(1→3)-β-d-GalpNAc-(1→ The d-GalpNAc residue was nonstoichiometrically substituted with a 4-O-methyl group (∼10%) or with a 4,6-O-(1-carboxy)-ethylidene residue (pyruvyl group) (∼10%).

Keywords: Fucose; Galactosamine; Lipopolysaccharide; Ochrobactrum cytisi; Pyruvyl.

MeSH terms

Carbohydrate Sequence
Fabaceae / microbiology*
Molecular Sequence Data
O Antigens / chemistry*
O Antigens / isolation & purification
Ochrobactrum / chemistry*
Ochrobactrum / physiology*
Symbiosis*

Substances

O Antigens