Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

Ling X Kong; Alexandra Perebikovsky; Jacob Moebius; Lawrence Kulinsky; Marc Madou

doi:10.1177/2211068215588456

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

J Lab Autom. 2016 Jun;21(3):323-55. doi: 10.1177/2211068215588456. Epub 2015 Jun 16.

Authors

Ling X Kong¹, Alexandra Perebikovsky², Jacob Moebius³, Lawrence Kulinsky⁴, Marc Madou⁵

Affiliations

¹ Department of Biomedical Engineering, University of California Irvine, CA, USA lxkong@uci.edu.
² Department of Physics and Astronomy, University of California Irvine, Irvine, CA, USA.
³ Department of Biomedical Engineering, University of California Irvine, CA, USA.
⁴ Department of Mechanical Engineering, University of California Irvine, Irvine, CA, USA.
⁵ Department of Biomedical Engineering, University of California Irvine, CA, USA Department of Mechanical Engineering, University of California Irvine, Irvine, CA, USA.

PMID: 26082453
DOI: 10.1177/2211068215588456

Abstract

The field of centrifugal microfluidics has experienced tremendous growth during the past 15 years, especially in applications such as lab-on-a-disc (LoD) diagnostics. The strength of LoD systems lies in its potential for development into fully integrated sample-to-answer analysis systems. This review highlights the technologies necessary to develop the next generation of these systems. In addition to outlining valving and other fluid-handling operations, we discuss the recent advances and future outlook in four categories of LoD processes: reagent storage, sample preparation, nucleic acid amplification, and analyte detection strategies.

Keywords: LoD; analytical; assay; centrifugal; microfluidics; point-of-care; sample-to-answer.

Publication types

Review

MeSH terms

Automation, Laboratory / methods*
Microfluidics / methods*
Microfluidics / trends
Molecular Diagnostic Techniques / methods*
Molecular Diagnostic Techniques / trends
Specimen Handling / methods
Specimen Handling / trends