Pteridic acid hydrate and pteridic acid C produced by StreStreptomyces pseudoverticillus YN17707 induce cell cycle arrest

Chin J Nat Med. 2015 Jun;13(6):467-70. doi: 10.1016/S1875-5364(15)30041-8.

Abstract

The present study aimed at identifying cell cycle inhibitors from the fermentation broth of Streptomyces pseudoverticillus YN17707. Activity-guided isolation was performed on tsFT210 cells. Compounds were isolated through various chromatographic methods and elucidated by spectroscopic analyses. Flow cytometry was used to evaluate the cell cycle inhibitory activities of the fractions and compounds. Two compounds were obtained and identified as pteridic acid hydrate (1) and pteridic acid C (2), which arrested the tsFT210 cells at the G0/G1 phase with the MIC values being 32.8 and 68.9 μmol·L(-1), respectively. These results provide a basis for future development of Compounds 1 and 2 as novel cell cycle inhibitors for cancer therapy.

Keywords: Cell cycle inhibitor; Flow cytometry; Pteridic acid; Spirocyclic polyketide; Streptomyces pseudoverticillus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Checkpoints / drug effects*
  • Cell Line
  • Heptanoic Acids / chemistry
  • Heptanoic Acids / isolation & purification*
  • Heptanoic Acids / pharmacology
  • Humans
  • Molecular Structure
  • Spiro Compounds / chemistry
  • Spiro Compounds / isolation & purification*
  • Spiro Compounds / pharmacology
  • Streptomyces / chemistry*

Substances

  • Heptanoic Acids
  • Spiro Compounds
  • pteridic acid A
  • pteridic acid C
  • pteridic acid hydrate