Functional Identification of OphR, an IclR Family Transcriptional Regulator Involved in the Regulation of the Phthalate Catabolic Operon in Rhodococcus sp. Strain DK17

Ki Young Choi; Beom Sik Kang; Myung Hee Nam; Woo Jun Sul; Eungbin Kim

doi:10.1007/s12088-015-0529-5

Functional Identification of OphR, an IclR Family Transcriptional Regulator Involved in the Regulation of the Phthalate Catabolic Operon in Rhodococcus sp. Strain DK17

Indian J Microbiol. 2015 Sep;55(3):313-8. doi: 10.1007/s12088-015-0529-5. Epub 2015 Apr 18.

Authors

Ki Young Choi¹, Beom Sik Kang², Myung Hee Nam³, Woo Jun Sul⁴, Eungbin Kim⁵

Affiliations

¹ Department of Systems Biology, Yonsei University, Seoul, 120-749 Korea ; Department of Systems Biotechnology, Chung-Ang University, Anseong, 456-756 Korea.
² School of Life Science and Biotechnology, Kyungpook National University, Daegu, 702-701 Korea.
³ Korea Basic Science Institute, Seoul Branch, Seoul, 136-701 Korea.
⁴ Department of Systems Biotechnology, Chung-Ang University, Anseong, 456-756 Korea.
⁵ Department of Systems Biology, Yonsei University, Seoul, 120-749 Korea.

Abstract

A putative gene for a transcriptional regulator (ophR) was detected near each copy of the duplicated phthalate-degrading operon of Rhodococcus sp. DK17. Sequence analysis and molecular modeling indicate that OphR belongs to the IclR family of transcriptional regulators and possesses the N-terminal DNA-binding and C-terminal effector-binding domains. DNA-binding assays demonstrate that OphR regulates the phthalate operon by binding to the ophA1-ophR intergenic region.

Keywords: Phthalate; Regulator; Rhodococcus sp. strain DK17; Transcription initiation.