Abstract
A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2-ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / immunology
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Ascorbate Peroxidases / chemistry
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Ascorbate Peroxidases / genetics
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Ascorbate Peroxidases / metabolism*
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Cell Line, Tumor
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Circular Dichroism
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism
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Hydrogen Peroxide / chemistry
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Immunoglobulin G / chemistry
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Immunoglobulin G / genetics
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Immunoglobulin G / metabolism*
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Mice
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Microscopy, Fluorescence
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Protein Structure, Tertiary
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Quartz Crystal Microbalance Techniques
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Surface Plasmon Resonance
Substances
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Antibodies
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Fluorescent Dyes
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Immunoglobulin G
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Recombinant Fusion Proteins
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Hydrogen Peroxide
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Ascorbate Peroxidases