Abstract
Validation of a high-throughput compatible 3D hyaluronic acid hydrogel coculture of cancer cells with stromal cells. The multilayered hyaluronic acid hydrogels improve drug screening predictability as evaluated with a panel of clinically relevant chemotherapeutics in both prostate and endometrial cancer cell lines compared to 2D culture.
Keywords:
3D culture; coculture; drug screening; high throughput; hyaluronic acid.
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / toxicity
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Automation
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Cell Culture Techniques
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Cell Line, Tumor
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Cell Survival / drug effects
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Coculture Techniques
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Drug Evaluation, Preclinical
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High-Throughput Screening Assays / instrumentation
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High-Throughput Screening Assays / methods*
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Humans
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Hyaluronic Acid / chemistry*
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Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry*
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Microscopy, Confocal
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Stromal Cells / cytology
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Stromal Cells / metabolism
Substances
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Antineoplastic Agents
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Hydrogel, Polyethylene Glycol Dimethacrylate
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Hyaluronic Acid