Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures

Adv Healthc Mater. 2015 Aug 5;4(11):1664-74. doi: 10.1002/adhm.201500258. Epub 2015 Jun 9.

Abstract

Validation of a high-throughput compatible 3D hyaluronic acid hydrogel coculture of cancer cells with stromal cells. The multilayered hyaluronic acid hydrogels improve drug screening predictability as evaluated with a panel of clinically relevant chemotherapeutics in both prostate and endometrial cancer cell lines compared to 2D culture.

Keywords: 3D culture; coculture; drug screening; high throughput; hyaluronic acid.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / toxicity
  • Automation
  • Cell Culture Techniques
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Coculture Techniques
  • Drug Evaluation, Preclinical
  • High-Throughput Screening Assays / instrumentation
  • High-Throughput Screening Assays / methods*
  • Humans
  • Hyaluronic Acid / chemistry*
  • Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry*
  • Microscopy, Confocal
  • Stromal Cells / cytology
  • Stromal Cells / metabolism

Substances

  • Antineoplastic Agents
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Hyaluronic Acid