Nuclear resonance vibrational spectroscopy (NRVS) of rubredoxin and MoFe protein crystals

Yisong Guo; Eric Brecht; Kristen Aznavour; Jay C Nix; Yuming Xiao; Hongxin Wang; Simon J George; Robert Bau; Stephen Keable; John W Peters; Michael W W Adams; Francis Jenney; Wolfgang Sturhahn; Ercan E Alp; Jiyong Zhao; Yoshitaka Yoda; Stephen P Cramer

doi:10.1007/s10751-012-0643-2

Nuclear resonance vibrational spectroscopy (NRVS) of rubredoxin and MoFe protein crystals

Hyperfine Interact. 2013 Dec;222(2 Suppl):77-90. doi: 10.1007/s10751-012-0643-2.

Authors

Yisong Guo¹, Eric Brecht², Kristen Aznavour³, Jay C Nix⁴, Yuming Xiao¹, Hongxin Wang⁵, Simon J George⁴, Robert Bau³, Stephen Keable², John W Peters², Michael W W Adams⁶, Francis Jenney⁷, Wolfgang Sturhahn⁸, Ercan E Alp⁸, Jiyong Zhao⁸, Yoshitaka Yoda⁹, Stephen P Cramer¹⁰

Affiliations

¹ Department of Applied Science, University of California, Davis, CA 95616.
² Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717.
³ Department of Chemistry, University of Southern California, Los Angeles, CA 90033.
⁴ Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720.
⁵ Department of Applied Science, University of California, Davis, CA 95616 ; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720.
⁶ Department of Chemistry, University of Georgia, Athens, GA 30602.
⁷ Georgia Campus, Philadelphia College of Osteopathic Medicine, Suwanee, GA 30024.
⁸ Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439.
⁹ JASRI, SPring-8, 1-1-1 Kouto, Mikazuki-cho, Sayo-gun, Hyogo 679-5198, Japan.
¹⁰ Department of Applied Science, University of California, Davis, CA 95616 ; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 ; Department of Chemistry, University of California, Davis, CA 95616.

Abstract

We have applied ⁵⁷Fe nuclear resonance vibrational spectroscopy (NRVS) for the first time to study the dynamics of Fe centers in Fe-S protein crystals, including oxidized wild type rubredoxin crystals from Pyrococcus furiosus, and the MoFe protein of nitrogenase from Azotobacter vinelandii. Thanks to the NRVS selection rule, selectively probed vibrational modes have been observed in both oriented rubredoxin and MoFe protein crystals. The NRVS work was complemented by extended X-ray absorption fine structure spectroscopy (EXAFS) measurements on oxidized wild type rubredoxin crystals from Pyrococcus furiosus. The EXAFS spectra revealed the Fe-S bond length difference in oxidized Pf Rd protein, which is qualitatively consistent with the X-ray crystal structure.

Keywords: 57Fe; EXAFS; Mössbauer; NRVS; nitrogenase; normal mode analysis; nuclear resonant scattering; nuclear resonant vibrational spectroscopy; rubredoxin; synchrotron radiation.

Abstract

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