Leaf oil bodies are subcellular factories producing antifungal oxylipins

Abstract

Oil bodies act as lipid storage compartments in plant cells. In seeds they supply energy for germination and early seedling growth. Oil bodies are also present in the leaves of many vascular plants, but their function in leaves has been poorly understood. Recent studies with oil bodies from senescent Arabidopsis thaliana leaves identified two enzymes, peroxygenase (CLO3) and α-dioxygenase (α-DOX), which together catalyze a coupling reaction to produce an antifungal compound (2-hydroxy-octadecanoic acid) from α-linolenic acid. Leaf oil bodies also have other enzymes including lipoxygenases, phospholipases, and triacylglycerol lipases. Hence, leaf oil bodies might function as intracellular factories to efficiently produce stable compounds via unstable intermediates by concentrating the enzymes and hydrophobic substrates.