Human serum biomarker detection based on a cascade signal amplification strategy by a DNA molecule machine

Chem Commun (Camb). 2015 Jul 11;51(54):10843-6. doi: 10.1039/c5cc03823e.

Abstract

A convenient method is presented that employs a DNA machine for protein biomarker detection. The detection limit is 400 times lower compared to the method without a DNA machine. This study provides a promising method that could realize most protein biomarker detections without the corresponding aptamers, using a DNA machine for signal amplification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry
  • Aptamers, Nucleotide / metabolism
  • Biomarkers / blood*
  • Biosensing Techniques
  • DNA / chemistry
  • DNA / metabolism*
  • Humans
  • Nucleic Acid Amplification Techniques
  • Prostate-Specific Antigen / blood
  • Spectrometry, Fluorescence

Substances

  • Aptamers, Nucleotide
  • Biomarkers
  • DNA
  • Prostate-Specific Antigen