Plate acoustic wave sensor for detection of small amounts of bacterial cells in micro-litre liquid samples

V I Anisimkin; I Е Kuznetsova; V V Kolesov; I I Pyataikin; V V Sorokin; D A Skladnev

doi:10.1016/j.ultras.2015.05.012

Plate acoustic wave sensor for detection of small amounts of bacterial cells in micro-litre liquid samples

Ultrasonics. 2015 Sep:62:156-9. doi: 10.1016/j.ultras.2015.05.012. Epub 2015 May 23.

Authors

V I Anisimkin¹, I Е Kuznetsova², V V Kolesov³, I I Pyataikin³, V V Sorokin⁴, D A Skladnev⁵

Affiliations

¹ Kotel'nikov Institute of Radio Engineering and Electronics of RAS, Moscow 125009, Russia. Electronic address: anis@cplire.ru.
² Kotel'nikov Institute of Radio Engineering and Electronics of RAS, Moscow 125009, Russia; MSTU MIREA, Moscow 119454, Russia.
³ Kotel'nikov Institute of Radio Engineering and Electronics of RAS, Moscow 125009, Russia.
⁴ Vinogradsky Institute of Microbiology RAS, Moscow 117312, Russia.
⁵ Vinogradsky Institute of Microbiology RAS, Moscow 117312, Russia. Electronic address: skladda@gmail.com.

PMID: 26049732
DOI: 10.1016/j.ultras.2015.05.012

Abstract

Ultrasonic acoustic waves propagating in thin piezoelectric plates with free faces are used for bacteria detection in micro-litre liquid samples deposited on one of the plate surface. The limits of the detection at normal conditions are as low as 0.04% for highly diluted rich cultural Luria-Bertani broth (LB-media) in distillate water, 0.07% for bacterial cells in distillate water, and 0.6% for bacterial cells in LB-media. For all analytes the most probable detection mechanism is the change in liquid conductivity. Because of no using any sorbent film the long-term stability of the detection is expected as very high.

Keywords: Piezoelectric plate; Plate acoustic wave; Sensor for detection of bacterial cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acoustics / instrumentation*
Biosensing Techniques / instrumentation*
Equipment Design
Manufactured Materials
Mycobacterium smegmatis*
Pseudomonas aeruginosa*
Sound