Effects of Lycium barbarum extract on production and immunomodulatory activity of the extracellular polysaccharopeptides from submerged fermentation culture of Coriolus versicolor

Fang-Yi Lin; Yiu-Kay Lai; Hao-Chen Yu; Nan-Yin Chen; Chi-Yue Chang; Hui-Chen Lo; Tai-Hao Hsu

doi:10.1016/j.foodchem.2008.02.023

Effects of Lycium barbarum extract on production and immunomodulatory activity of the extracellular polysaccharopeptides from submerged fermentation culture of Coriolus versicolor

Food Chem. 2008 Sep 15;110(2):446-53. doi: 10.1016/j.foodchem.2008.02.023. Epub 2008 Feb 15.

Authors

Fang-Yi Lin¹, Yiu-Kay Lai², Hao-Chen Yu¹, Nan-Yin Chen³, Chi-Yue Chang¹, Hui-Chen Lo⁴, Tai-Hao Hsu⁵

Affiliations

¹ Department of Bioindustry Technology, Da-Yeh University, Changhua County 51591, Taiwan.
² Department of Bioresources, Da-Yeh University, Changhua County 51591, Taiwan; Institute of Biotechnology and Department of Life Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan.
³ Department of Food Nutrition, Chung-Hwa University of Medical Technology, Tainan Hsien, 71703, Taiwan.
⁴ Department of Bioscience Technology, Chang-Jung Christian University, Tainan 71101, Taiwan; Department of Medical Education and Research, Changhua Christian Hospital, Changhua 50006, Taiwan.
⁵ Department of Bioindustry Technology, Da-Yeh University, Changhua County 51591, Taiwan. Electronic address: th4420@gmail.com.

PMID: 26049238
DOI: 10.1016/j.foodchem.2008.02.023

Abstract

Polysaccharopeptides (PSPs) from Coriolus versicolor have been used as immunomodulatory and anticancer agents. However, most studies have concentrated on the mycelial PSPs and not those in the fermented broth. On the other hand, Lycium barbarum fruit has been used as a traditional Chinese herbal medicine for two millennia. Its extract contains various nutrients, minerals, and also polysaccharide-protein complexes, which are proven to be bioactive. Herein we report the effects of L. barbarum fruit extract on the mycelial growth and extracellular PSP (ePSP) production of C. versicolor LH1 by using a submerged fermentation process in 20l fermenters. Fermentation production of C. versicolor biomass and its ePSP were augmented in the presence of L. barbarum extract. The ePSP such obtained differs from those obtained with normal culture medium in terms of simple sugar composition and protein content but shows similar overall chemical structures as analyzed by Fourier transformed infrared spectroscopy. Moreover, the ePSP from C. versicolor cultured with supplementary L. barbarum extract exhibits significant immunomodulatory activity as judged by its effects on the production of nitric oxide and several cytokines by murine RAW264.7 macrophages.

Keywords: Coriolus versicolor; Extracellular polysaccharopeptides; Immunomdulatory activity; Lycium barbarum extract; Macrophages.