Cell lysis induced by membrane-damaging detergent saponins from Quillaja saponaria

Enzyme Microb Technol. 2015 Jul-Aug:75-76:44-8. doi: 10.1016/j.enzmictec.2015.04.007. Epub 2015 May 2.

Abstract

This paper presents the results of a study to determine the effect of Quillaja saponaria saponins on the lysis of industrial yeast strains. Cell lysis induced by saponin from Q. saponaria combined with the plasmolysing effect of 5% NaCl for Saccharomyces cerevisiae, Kluyveromyces marxianus yeasts biomass was conducted at 50 °C for 24-48 h. Membrane permeability and integrity of the yeast cells were monitored using fluorescent techniques and concentrations of proteins, free amino nitrogen (FAN) and free amino acids in resulting lysates were analyzed. Protein release was significantly higher in the case of yeast cell lysis promoted with 0.008% Q. saponaria and 5% NaCl in comparison to plasmolysis triggered by NaCl only.

Keywords: Cell lysis; Saponin; Yeast; Yeast extract.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / metabolism
  • Ammonia / metabolism
  • Autolysis
  • Cell Membrane / drug effects
  • Cell Membrane Permeability / drug effects
  • Detergents / pharmacology*
  • Industrial Microbiology
  • Kluyveromyces / drug effects
  • Kluyveromyces / metabolism
  • Proteolysis / drug effects
  • Quillaja / chemistry*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / metabolism
  • Saponins / pharmacology*

Substances

  • Amino Acids
  • Detergents
  • Saponins
  • Ammonia