Authentication of "Cereza del Jerte" sweet cherry varieties by free zone capillary electrophoresis (FZCE)

Manuel J Serradilla; Alberto Martín; Emilio Aranda; Alejandro Hernández; María J Benito; Margarita Lopez-Corrales; María de Guía Córdoba

doi:10.1016/j.foodchem.2008.03.084

Authentication of "Cereza del Jerte" sweet cherry varieties by free zone capillary electrophoresis (FZCE)

Food Chem. 2008 Nov 15;111(2):457-61. doi: 10.1016/j.foodchem.2008.03.084. Epub 2008 Apr 6.

Authors

Manuel J Serradilla¹, Alberto Martín², Emilio Aranda³, Alejandro Hernández³, María J Benito³, Margarita Lopez-Corrales¹, María de Guía Córdoba³

Affiliations

¹ Hortofruticultura, Centro de Investigación Finca La Orden-Valdesequera, Junta de Extremadura, Autovía Madrid-Lisboa s/n, Guadajira, 06187 Badajoz, Spain.
² Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura,(1) Ctra. de Cáceres s/n, 06071 Badajoz, Spain. Electronic address: amartin@unex.es.
³ Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura,(1) Ctra. de Cáceres s/n, 06071 Badajoz, Spain.

PMID: 26047450
DOI: 10.1016/j.foodchem.2008.03.084

Abstract

The purpose of this work was to develop a procedure based on protein analysis by free zone capillary electrophoresis (FZCE) that can be used as an alternative to other methods in the determination of sweet cherry varieties for the authentication of "Cereza del Jerte". Two autochthonous varieties of sweet cherry type "Picota", 'Ambrunés' and 'Pico Negro', and the foreign variety 'Sweetheart' were used in the study. Two protocols for extracting the methanol-soluble proteins were tested. On the basis of the results, direct evaporation with nitrogen of a methanol extract was included in the extraction protocol for routine analysis. This method was found to give excellent repeatability of the corrected migration time (CMT), and showed greater effectiveness in discriminating sweet cherry varieties than the SDS-PAGE technique. Three peaks found in the FZCE electropherograms were investigated as a basis for discriminating between varieties. In addition, the FZCE analysis of methanol-soluble proteins provides information about the physico-chemical parameters relevant to the sensorial quality of the sweet cherries.

Keywords: Authentication; FZCE; Protein profile; Sweet cherry.