Abstract
The ankyrin repeat domain 49 (ANKRD49) is an evolutionarily conserved protein highly expressed in testes. However, the function of ANKRD49 in spermatogenesis is unknown. In this study, we found that ANKRD49 resides primarily in nucleus of spermatogonia, spermatocytes and round spermatids. ANKRD49 overexpression augments starvation-induced autophagy in male germ GC-1 cells whereas shRNA knockdown of ANKRD49 attenuates the autophagy. Inhibition of NF-κB pathway by its inhibitors or p65 siRNA prevents the ANKRD49-dependent autophagy augmentation, demonstrating that ANKRD49 enhances autophagy via NF-κB pathway. Our findings suggest that ANKRD49 plays an important role in spermatogenesis via promotion of autophagy-dependent survival.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Ankyrin Repeat*
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Autophagy* / drug effects
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Cell Line
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Culture Media, Serum-Free / pharmacology*
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Gene Expression Regulation, Developmental / drug effects
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Gene Knockdown Techniques
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Male
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Mice, Inbred BALB C
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Molecular Sequence Data
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Muscle Proteins / chemistry
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Muscle Proteins / genetics
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Muscle Proteins / metabolism*
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NF-kappa B / metabolism*
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Prophase / drug effects
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Signal Transduction / drug effects*
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Spermatogenesis / drug effects
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Spermatozoa / drug effects
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Spermatozoa / metabolism
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Testis / drug effects
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Testis / metabolism
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Time Factors
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Transcription, Genetic / drug effects
Substances
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Culture Media, Serum-Free
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Muscle Proteins
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NF-kappa B
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ankyrin repeat domain 49 protein, mouse
Grants and funding
This study was supported by the Scientific Research Foundation for Returned Scholars of Shanxi Province (2011‑043, 2010‑677) and the Biology Postdoctoral Mobile Research Station of Shanxi Medical University.