Identification of innovative potential quality markers in rocket and melon fresh-cut produce

Abstract

Ready-to-eat fresh cut produce are exposed to pre- and postharvest abiotic stresses during the production chain. Our work aimed to identify stress responsive genes as new molecular markers of quality that can be widely applied to leaves and fruits and easily determined at any stage of the production chain. Stress responsive genes associated with quality losses were isolated in rocket and melon fresh-cut produce and their expression levels analyzed by quantitative real time PCR (qRT-PCR) at different time points after harvest at 20 °C and 4 °C. qRT-PCR results were supported by correlation analysis with physiological and biochemical determinations evaluated at the same conditions such as chlorophyll a fluorescence indices, total, reducing sugars, sucrose, ethylene, ascorbic acid, lipid peroxidation and reactive oxygen species. In both species the putative molecular markers increased their expression soon after harvest suggesting a possible use as novel and objective quality markers of fresh-cut produces.

Keywords: Abiotic stress; Ascorbic acid (PubChem CID: 54670067); Chlorophyll (PubChem CID: 6477652); Ethylene (PubChem CID: 6325); Fresh-cut vegetables; Fructose (PubChem CID: 53782691); Galactose (PubChem CID: 439357); Glucose (PubChem CID: 53782692); Hydrogen peroxide (PubChem CID: 784); Malondialdehyde (PubChem CID: 10964); Postharvest; Production chain; Senescence; Sucrose (PubChem CID: 5988); Superoxide anion (PubChem CID: 5359597).