Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix

Talita F B Souza; Silmara S Sakamoto; Gabriel T N M Ferreira; Roberto Gameiro; Marcia Marinho; Alexandre L de Andrade; Tereza C Cardoso

doi:10.4142/jvs.2015.16.3.381

Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix

J Vet Sci. 2015;16(3):381-4. doi: 10.4142/jvs.2015.16.3.381. Epub 2015 Jun 3.

Authors

Talita F B Souza¹, Silmara S Sakamoto, Gabriel T N M Ferreira, Roberto Gameiro, Marcia Marinho, Alexandre L de Andrade, Tereza C Cardoso

Affiliation

¹ Laboratory of Animal Virology and Cell Culture, College of Veterinary Medicine, UNESP-Universidade Estadual Paulista, Aracatuba, SP 16050-680, Brazil.

Abstract

Canine mesenchymal cells (MSCs) derived from Wharton's jelly were co-cultured, then supplemented or not supplemented with platelet rich plasma (PRP) and demineralized bone matrix (DBM) to verify osteogenic differentiation. Osteoblastic differentiation followed by mineralized bone matrix production was found to be significantly higher (p < 0.05) when MSCs were associated with PRP/DBM in culture after 14-21-days of induction. Osteopontin and osteocalcin gene expression were significantly superior (p < 0.05) under the same culture conditions after 21 days of observation. In conclusion, addition of PRP to DBM co-cultured with MSCs successfully induced osteogenesis in vitro.

Keywords: bone; mesenchymal stem cells; platelet-rich plasma; umbilical cord.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Demineralization Technique / veterinary
Bone Matrix / metabolism*
Cell Differentiation
Cells, Cultured
Coculture Techniques / veterinary
Dogs
Mesenchymal Stem Cells / metabolism*
Osteogenesis*
Platelet-Rich Plasma / metabolism*
Umbilical Cord / metabolism