Cationic star copolymers based on β-cyclodextrins for efficient gene delivery to mouse embryonic stem cell colonies

Chem Commun (Camb). 2015 Jul 11;51(54):10815-8. doi: 10.1039/c5cc03686k.

Abstract

A cationic star copolymer with a β-cyclodextrin core was developed for nonviral gene transfer to mouse embryonic stem cells (mESCs). The copolymer comprises poly(2-dimethyl aminoethyl methacrylate) as the cationic component and poly(2-hydroxyethyl methacrylate) as the non-toxic stealth component. These materials have very low toxicity and show highly efficient transfection to mESC colonies.

MeSH terms

  • Animals
  • Cations / chemistry
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Mice
  • Microscopy, Fluorescence
  • Mouse Embryonic Stem Cells / cytology
  • Mouse Embryonic Stem Cells / metabolism*
  • Plasmids / genetics
  • Plasmids / metabolism
  • Polymers / chemistry*
  • Transfection
  • beta-Cyclodextrins / chemistry*

Substances

  • Cations
  • Polymers
  • beta-Cyclodextrins
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins