A new method for measuring osteoclast formation by electrical impedance

Haruka Emori; Shinichi Iwai; Kakei Ryu; Hitoshi Amano; Takehiko Sambe; Takahiro Kobayashi; Tatsunori Oguchi; Kiyoshi Ohura; Katsuji Oguchi

doi:10.1016/j.jphs.2015.05.002

A new method for measuring osteoclast formation by electrical impedance

J Pharmacol Sci. 2015 Jun;128(2):87-91. doi: 10.1016/j.jphs.2015.05.002. Epub 2015 May 15.

Authors

Haruka Emori¹, Shinichi Iwai², Kakei Ryu³, Hitoshi Amano⁴, Takehiko Sambe³, Takahiro Kobayashi³, Tatsunori Oguchi³, Kiyoshi Ohura⁵, Katsuji Oguchi³

Affiliations

¹ Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Department of Orthopaedic Surgery, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
² Department of Healthcare and Regulatory Sciences, Showa University School of Pharmacy, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
³ Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
⁴ Department of Pharmacology, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata, Osaka 573-1121, Japan. Electronic address: amano@cc.osaka-dent.ac.jp.
⁵ Department of Pharmacology, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata, Osaka 573-1121, Japan.

PMID: 26032840
DOI: 10.1016/j.jphs.2015.05.002

Abstract

Osteoclasts are important target cells for osteoporosis treatment. Recently, a real-time cell analysis (RTCA) system was developed to observe cell morphology and adhesion; however, the use of RTCA to study osteoclastogenesis has not been reported. Here, we investigated whether osteoclast formation could be monitored in real-time using RTCA. The cell index determined via electrical impedance using RTCA, and the number of osteoclasts exhibited a significant positive correlation. RTCA was useful for determining the effect of (-)-epigallocatechin-3-gallate on the inhibition of bone resorption. We established a new method of measuring osteoclast formation in real-time using RTCA.

Keywords: Cell morphology; Osteoclast; Real-time cell analyzing system.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bone Resorption / pathology
Bone Resorption / prevention & control
Catechin / analogs & derivatives
Catechin / pharmacology
Cell Differentiation
Cells, Cultured
Computer Systems*
Cytological Techniques / methods*
Electric Impedance*
Humans
Macrophage Colony-Stimulating Factor
Osteoclasts / cytology*
RANK Ligand

Substances

RANK Ligand
Macrophage Colony-Stimulating Factor
Catechin
epigallocatechin gallate