Synthesis and in vitro activity of platinum(II) complexes of two fluorenylspirohydantoins against a human tumour cell line

Petja Marinova; Marin Marinov; Maria Kazakova; Yana Feodorova; Plamen Penchev; Victoria Sarafian; Neyko Stoyanov

doi:10.1080/13102818.2014.910363

Synthesis and in vitro activity of platinum(II) complexes of two fluorenylspirohydantoins against a human tumour cell line

Biotechnol Biotechnol Equip. 2014 Mar 4;28(2):316-321. doi: 10.1080/13102818.2014.910363. Epub 2014 Jul 10.

Authors

Petja Marinova¹, Marin Marinov², Maria Kazakova³, Yana Feodorova³, Plamen Penchev¹, Victoria Sarafian³, Neyko Stoyanov⁴

Affiliations

¹ Faculty of Chemistry, University of Plovdiv , Plovdiv , Bulgaria.
² Faculty of Plant protection and Agroecology, Agricultural University , Plovdiv , Bulgaria.
³ Depatment of Biology, Medical University , Plovdiv , Bulgaria.
⁴ University of Ruse , Razgrad , Bulgaria.

Abstract

This paper presents a method for synthesis and cytotoxicity of new platinum(II) complexes of (9'-fluorene)-spiro-5-hydantoin (L1) and (9'-fluorene)-spiro-5-(2-thiohydantoin) (L2). The new obtained complexes were studied by elemental analysis: ultraviolet-visible, attenuated total reflection Fourier transform infrared (ATR-FTIR), and ¹H- and ¹³C-NMR for Pt(II) compounds and additionally Raman spectroscopy for free ligands. Based on the experimental data, the most probable structure of the complexes is suggested. In the present study, we have examined cytotoxic activity of (9'-fluorene)-spiro-5-hydantoin (L1) and (9'-fluorene)-spiro-5-(2-thiohydantoin) (L2) and their Pt(II) complexes on the retinoblastoma cell line WERI-Rb-1.

Keywords: (9′-fluorene)-spiro-5-(2-thiohydantoin); (9′-fluorene)-spiro-5-hydantoin; cytotoxic/antiproliferative effects; fluorenylspirohydantoins; metal complexes.

Grants and funding

This work was supported by the National Science Fund (NSF) [grant number DMU 02/11] and [grant number DFNI BO1/0014]. The publication of this work was supported by ‘Science and Business’ of the Operative Programme ‘Development of Human Resources’ of the European Social Fund (Contract D02-159/14.02.2013 index P-6-33/13) [grant number BG051PO001-3.3.05-0001].