Next-generation sequencing yields the complete mitochondrial genome of the longfang moray, Enchelynassa canina (Anguilliformes: Muraenidae)

Kar-Hoe Loh; Kwang-Tsao Shao; Hong-Ming Chen; Ching-Hung Chen; Poh-Leong Loo; Amy Then Yee Hui; Phaik-Eem Lim; Ving-Ching Chong; Kang-Ning Shen; Chung-Der Hsiao

doi:10.3109/19401736.2015.1030629

Next-generation sequencing yields the complete mitochondrial genome of the longfang moray, Enchelynassa canina (Anguilliformes: Muraenidae)

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2431-2. doi: 10.3109/19401736.2015.1030629. Epub 2015 May 27.

Authors

Affiliations

¹ a Institute of Ocean and Earth Sciences, University of Malaya , Kuala Lumpur , Malaysia .
² b Biodiversity Research Center, Academia Sinica , Taipei , Taiwan .
³ c Department of Aquaculture , National Taiwan Ocean University , Keelung , Taiwan .
⁴ d WeThink Biotech INC. , Taoyuan , Taiwan .
⁵ e Institute of Biological Sciences, University of Malaya , Kuala Lumpur , Malaysia .
⁶ f Center of Excellence for the Oceans, National Taiwan Ocean University , Keelung , Taiwan , and.
⁷ g Department of Bioscience Technology , Chung Yuan Christian University , Chung-Li , Taiwan.

PMID: 26016872
DOI: 10.3109/19401736.2015.1030629

Abstract

In this study, the complete mitogenome sequence of the longfang moray, Enchelynassa canina (Anguilliformes: Muraenidae) has been sequenced by the next-generation sequencing method. The length of the assembled mitogenome is 16,592 bp, which includes 13 protein coding genes, 22 transfer RNAs, and 2 ribosomal RNAs genes. The overall base composition of longfang moray is 28.4% for A, 28.0% for C, 18.4% for G, 25.1% for T, and show 82% identities to Kidako moray, Gymnothorax kidako. The complete mitogenome of the longfang moray provides an essential and important DNA molecular data for further phylogeography and evolutionary analysis for moray eel phylogeny.

Keywords: Longfang moray; mitogenome; next-generation sequencing.

MeSH terms

Animals
Base Composition
Eels / classification*
Eels / genetics*
Evolution, Molecular
Genes, Mitochondrial
Genome Size
Genome, Mitochondrial*
High-Throughput Nucleotide Sequencing*
Open Reading Frames
Phylogeny
Sequence Analysis, DNA
Whole Genome Sequencing