Deepwater fishes from the Amundsen Sea, d'Urville Sea, Ross Sea, Cooperation Sea, Weddell Sea, and open waters of the South-West part of the Pacific sector of the Antarctic were examined. Fourteen species of trematodes were found. Muraenolepitrema magnatestis Gaevskaya et Rodjuk, 1988 and Helicometrina sp. were found in the Antarctic for the first time. Muraenolepitrema magnatestis was redescribed and basing on this, diagnosis of the genus Muraenolepitrema was amended. Muraenolepitrema magnatestis possesses the uroproct and glandular cells that are loosely arranged around the external seminal vesicle (without a membranous sac).