Determining the geometry of oligomers of the human epidermal growth factor family on cells with <10 nm resolution

Biochem Soc Trans. 2015 Jun;43(3):309-14. doi: 10.1042/BST20140318.

Abstract

There is a limited range of methods available to characterize macromolecular organization in cells on length scales from 5-50 nm. We review methods currently available and show the latest results from a new single-molecule localization-based method, fluorophore localization imaging with photobleaching (FLImP), using the epidermal growth factor (EGF) receptor (EGFR) as an example system. Our measurements show that FLImP is capable of achieving spatial resolution in the order of 6 nm.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Epidermal Growth Factor / chemistry*
  • ErbB Receptors / chemistry*
  • Fluorescent Dyes / chemistry
  • Humans
  • Macromolecular Substances / chemistry*
  • Protein Multimerization

Substances

  • Fluorescent Dyes
  • Macromolecular Substances
  • Epidermal Growth Factor
  • EGFR protein, human
  • ErbB Receptors