Peptidoglycan diversity and anti-inflammatory capacity in Lactobacillus strains

Carbohydr Polym. 2015 Sep 5:128:130-7. doi: 10.1016/j.carbpol.2015.04.026. Epub 2015 Apr 22.

Abstract

Lactobacillus species are potential probiotic bacteria for humans because of their capacity to improve certain biological functions in the host's immune system. In this study, we focused on three peptidoglycans (PGNs) derived from different Lactobacillus strains and investigated each PGN's anti-inflammatory capacity. Each PGN was analyzed using HPLC, MALDI-TOF/TOF MS and FTIR. All three PGNs displayed a β-1,4-linked N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) structure with some modifications in the polypeptides at the end of the MurNAc residue. In a new insight, we found that PGNs inhibit the release of inflammatory cytokines in LPS-induced RAW 264.7 cells; a capacity that may be related to the TLR-4 pathway. The goal for exploring PGN diversity in Lactobacillus strains is to better understand the potential use of Lactobacillus PGNs in food and pharmaceutical applications.

Keywords: Anti-inflammatory capacity; DHB (PubChem CID: 24101); FITC (PubChem CID: 18730); HT-29 cells; Lactobacillus strains; N-acetylglucosamine (PubChem CID: 11861101); N-acetylmuramic acid (PubChem CID: 12917652); PGN diversity; Peptidoglycan (PubChem CID: 9816401); RAW 264.7 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Cell Line
  • Cytokines / metabolism
  • HT29 Cells
  • Humans
  • Lactobacillus*
  • Lipopolysaccharides / pharmacology
  • Mice
  • Peptidoglycan / pharmacology*
  • Phagocytosis
  • Toll-Like Receptor 4 / metabolism

Substances

  • Anti-Inflammatory Agents
  • Cytokines
  • Lipopolysaccharides
  • Peptidoglycan
  • Tlr4 protein, mouse
  • Toll-Like Receptor 4