Effect of olive leaf extract treatment on doxorubicin-induced cardiac, hepatic and renal toxicity in rats

Alkın Kumral; Murat Giriş; Merva Soluk-Tekkeşin; Vakur Olgaç; Semra Doğru-Abbasoğlu; Ümit Türkoğlu; Müjdat Uysal

doi:10.1016/j.pathophys.2015.04.002

Effect of olive leaf extract treatment on doxorubicin-induced cardiac, hepatic and renal toxicity in rats

Pathophysiology. 2015 Jun;22(2):117-23. doi: 10.1016/j.pathophys.2015.04.002. Epub 2015 May 7.

Authors

Alkın Kumral¹, Murat Giriş², Merva Soluk-Tekkeşin³, Vakur Olgaç³, Semra Doğru-Abbasoğlu¹, Ümit Türkoğlu¹, Müjdat Uysal⁴

Affiliations

¹ Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa, 34093 Istanbul, Turkey.
² Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey.
³ Oncology Institute, Istanbul University, Department of Pathology, Istanbul, Turkey.
⁴ Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa, 34093 Istanbul, Turkey. Electronic address: m.mujdatuysal@hotmail.com.

PMID: 26002558
DOI: 10.1016/j.pathophys.2015.04.002

Abstract

Doxorubicin (DOX) is known to increase in oxidative stress in several organs. Olive leaf extract (OLE) has potent antioxidant effects; therefore, we evaluated the ability of OLE to reduce DOX-induced toxicity in the heart, liver, and kidneys of rats. DOX (30mg/kg; i.p.) was administered to rats, which were sacrificed 4 days after DOX. The rats received OLE (6 and 12mL/L in drinking water) for 12 days. Serum cardiac troponin I (cTnI) levels, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activities, urea and creatinine levels, as well as prooxidant and antioxidant status in organs were measured. DOX was found to increase serum markers that indicate tissue injury, malondialdehyde (MDA), diene conjugate (DC), and protein carbonyl (PC) levels, and to decrease glutathione (GSH) levels in organs. Histopathologic changes were also evaluated. OLE, especially OLE 1000, led to decreases in serum cTnI and urea levels, ALT and AST activities, and amelioration in histopathologic findings. Decreases in MDA, DC, and PC, and increases in GSH levels were observed in organs of DOX-treated rats due to OLE. We conclude that OLE treatment may be effective in decreasing DOX-induced cardiac, hepatic and renal oxidative stress and injury.

Keywords: Doxorubicin; Olive leaf extract; Organs; Oxidative stress; Rat.