Toward Whole-Transcriptome Editing with CRISPR-Cas9

Dirk Heckl; Emmanuelle Charpentier

doi:10.1016/j.molcel.2015.05.016

Toward Whole-Transcriptome Editing with CRISPR-Cas9

Mol Cell. 2015 May 21;58(4):560-2. doi: 10.1016/j.molcel.2015.05.016.

Authors

Dirk Heckl¹, Emmanuelle Charpentier²

Affiliations

¹ Department of Pediatric Hematology and Oncology, Hannover Medical School, Hannover 30625, Germany. Electronic address: heckl.dirk@mh-hannover.de.
² Department of Regulation in Infection Biology, Helmholtz Centre for Infection Research, Braunschweig 38124, Germany; The Laboratory for Molecular Infection Medicine Sweden (MIMS), Department of Molecular Biology, Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå 90187, Sweden; Department of Regulation in Infection Biology, Hannover Medical School, Hannover 30625, Germany. Electronic address: emmanuelle.charpentier@helmholtz-hzi.de.

PMID: 26000839
DOI: 10.1016/j.molcel.2015.05.016

Abstract

Targeted regulation of gene expression holds huge promise for biomedical research. In a series of recent publications (Gilbert et al., 2014; Konermann et al., 2015; Zalatan et al., 2015), sophisticated, multiplex-compatible transcriptional activator systems based on the CRISPR-Cas9 technology and genome-scale libraries advance the field toward whole-transcriptome control.

Publication types

Comment

MeSH terms

CRISPR-Cas Systems / genetics*
Gene Expression*
Genetic Engineering / methods*
Genetic Techniques*
Genome, Human / genetics*
Humans
Melanoma / genetics*
Transcription, Genetic*
Transcriptional Activation / genetics*