Synthetic indole Mannich bases: Their ability to modulate in vitro cellular immunity

M Ahmed Mesaik; Khalid Mohammed Khan; Fazal Rahim; Muhammad Taha; Syed Moazzam Haider; Shahnaz Perveen; Ahmed Shukralla Khalid; Omer M Abdalla; Samreen Soomro; Wolfgang Voelter

doi:10.1016/j.bioorg.2015.05.003

Synthetic indole Mannich bases: Their ability to modulate in vitro cellular immunity

Bioorg Chem. 2015 Jun:60:118-22. doi: 10.1016/j.bioorg.2015.05.003. Epub 2015 May 8.

Authors

Affiliations

¹ Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan; Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia. Electronic address: mmesaik@hotmail.com.
² H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan. Electronic address: hassaan2@super.net.pk.
³ Department of Chemistry, Hazara University, Mansehra, Khyber Pukhthunkhwa, Pakistan.
⁴ Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA (UiTM), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor D.E., Malaysia; Faculty of Applied Science, UiTM, 40450 Shah Alam, Selangor, Malaysia.
⁵ H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
⁶ PCSIR Laboratories Complex, Karachi, Shahrah-e-Dr. Salimuzzaman Siddiqui, Karachi 75280, Pakistan.
⁷ Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan; Institute of Radiobiology, Ministry of Science and Technology, P.O. Box 3001, Khartoum, Sudan.
⁸ Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
⁹ Interfakultäres Institut für Biochemie, Universität Tübingen, Hoppe-Seyler-Str. 4, 72076 Tübingen, Germany.

PMID: 26000491
DOI: 10.1016/j.bioorg.2015.05.003

Abstract

The synthetic indole Mannich bases 1-13 have been investigated for their ability to modulate immune responses measured in vitro. These activities were based on monitoring their affects on T-lymphocyte proliferation, reactive oxygen species (ROS), IL (interleukin)-2, IL-4, and nitric oxide production. Compound 5 was found to be the most potent immunomodulator in this context. Four of the synthesized compounds, 5, 11, 12, and 13, have significant potent inhibitory effects on T-cell proliferation, IL-4, and nitric oxide production. However, none of the thirteen indole compounds exerted any activity against ROS production.

Keywords: Cytokine inhibition; Indole Mannich base; Nitric oxide; ROS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Animals
Cattle
Cell Line
Cell Proliferation / drug effects
Cytokines / immunology
Humans
Immunity, Cellular / drug effects*
Immunologic Factors / chemistry*
Immunologic Factors / pharmacology*
Indoles / chemistry*
Indoles / pharmacology*
Mannich Bases / chemistry*
Mannich Bases / pharmacology*
Nitric Oxide / immunology
Reactive Oxygen Species / immunology
T-Lymphocytes / drug effects
T-Lymphocytes / immunology

Substances

Cytokines
Immunologic Factors
Indoles
Mannich Bases
Reactive Oxygen Species
Nitric Oxide
indole