Multitarget PCR Assay for Direct Detection of Penicillinase-Producing Neisseria gonorrhoeae for Enhanced Surveillance of Gonococcal Antimicrobial Resistance

Cameron Buckley; Ella Trembizki; Robert W Baird; Marcus Chen; Basil Donovan; Kevin Freeman; Namraj Goire; Rebecca Guy; Monica M Lahra; David Regan; David M Whiley

doi:10.1128/JCM.00540-15

Multitarget PCR Assay for Direct Detection of Penicillinase-Producing Neisseria gonorrhoeae for Enhanced Surveillance of Gonococcal Antimicrobial Resistance

J Clin Microbiol. 2015 Aug;53(8):2706-8. doi: 10.1128/JCM.00540-15. Epub 2015 May 20.

Authors

Affiliations

¹ Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Queensland, Australia The University of Queensland, Brisbane, Queensland, Australia.
² Microbiology Laboratory, Pathology Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia.
³ Melbourne Sexual Health Centre, Alfred Health, Carlton Victoria, Australia Central Clinical School, Monash University, Victoria, Australia.
⁴ Kirby Institute, University of New South Wales, Sydney, NSW, Australia Sydney Sexual Health Centre, Sydney Hospital, Sydney, NSW, Australia.
⁵ WHO Collaborating Centre for STD, Microbiology Department, South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, New South Wales, Australia Nepean Clinical School, University of Sydney, Kingswood, New South Wales, Australia.
⁶ Kirby Institute, University of New South Wales, Sydney, NSW, Australia.
⁷ WHO Collaborating Centre for STD, Microbiology Department, South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, New South Wales, Australia.
⁸ Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Queensland, Australia The University of Queensland, Brisbane, Queensland, Australia d.whiley@uq.edu.au.

Abstract

A multitarget PCR was developed for the direct detection of penicillinase-producing Neisseria gonorrhoeae (PPNG). The assay was validated by testing 342 PPNG isolates and 415 clinical samples. The method is suitable for routine detection of PPNG strains. Its multitarget approach reduces the potential for false-negative results caused by sequence variations.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Drug Resistance, Bacterial*
Epidemiological Monitoring
Gonorrhea / epidemiology
Gonorrhea / microbiology*
Humans
Neisseria gonorrhoeae / drug effects
Neisseria gonorrhoeae / enzymology*
Neisseria gonorrhoeae / genetics
Penicillinase / genetics*
Polymerase Chain Reaction / methods*

Substances

Penicillinase