Structure of the O-polysaccharide of Escherichia coli O87

Evelina L Zdorovenko; Alla K Golomidova; Nikolai S Prokhorov; Alexander S Shashkov; Lei Wang; Andrei V Letarov; Yuriy A Knirel

doi:10.1016/j.carres.2015.04.014

Structure of the O-polysaccharide of Escherichia coli O87

Carbohydr Res. 2015 Aug 14:412:15-8. doi: 10.1016/j.carres.2015.04.014. Epub 2015 May 1.

Authors

Evelina L Zdorovenko¹, Alla K Golomidova², Nikolai S Prokhorov², Alexander S Shashkov³, Lei Wang⁴, Andrei V Letarov⁵, Yuriy A Knirel³

Affiliations

¹ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia. Electronic address: zdorovenkoe@mail.ru.
² S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, 117312 Moscow, Russia.
³ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
⁴ TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, China.
⁵ S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, 117312 Moscow, Russia; Moscow Institute of Physics and Technology State University, 141700 Dolgoprudny, Moscow Region, Russia.

PMID: 25988496
DOI: 10.1016/j.carres.2015.04.014

Abstract

The following structure of the O-polysaccharide of Escherichia coli HS1/2 serving as a primary receptor for bacteriophage DT57-12 was elucidated by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy: This structure is shared by E. coli O87 type strain. Putatively assigned functions of genes in the O-antigen gene cluster of E. coli O87 are consistent with the O-polysaccharide structure established.

Keywords: Bacterial polysaccharide structure; Escherichia coli O87; Lipopolysaccharide; O-Antigen gene cluster; O-Specific polysaccharide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Escherichia coli / chemistry*
Escherichia coli / genetics
Magnetic Resonance Imaging
O Antigens / chemistry*

Substances

O Antigens