Segregation versus colocalization: orthogonally functionalized binary micropatterned substrates regulate the molecular distribution in focal adhesions

Judith Guasch; Bert Conings; Stefanie Neubauer; Florian Rechenmacher; Karen Ende; Claudio G Rolli; Christian Kappel; Viktoria Schaufler; Alexandre Micoulet; Horst Kessler; Hans-Gerd Boyen; Elisabetta Ada Cavalcanti-Adam; Joachim P Spatz

doi:10.1002/adma.201500900

Segregation versus colocalization: orthogonally functionalized binary micropatterned substrates regulate the molecular distribution in focal adhesions

Adv Mater. 2015 Jul 1;27(25):3737-47. doi: 10.1002/adma.201500900. Epub 2015 May 15.

Authors

Judith Guasch^{1

2}, Bert Conings³, Stefanie Neubauer^{1

4}, Florian Rechenmacher⁴, Karen Ende¹, Claudio G Rolli^{1

2}, Christian Kappel¹, Viktoria Schaufler^{1

2}, Alexandre Micoulet^{1

2}, Horst Kessler⁴, Hans-Gerd Boyen³, Elisabetta Ada Cavalcanti-Adam^{1

2}, Joachim P Spatz^{1

2}

Affiliations

¹ Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.
² Department of Biophysical Chemistry, University of Heidelberg INF 253, Heidelberg, D-69120, Germany.
³ Institute for Materials Research, Wetenschapspark 1, Diepenbeek, B-3590, Belgium.
⁴ Institute for Advanced Study (IAS) and Center of Integrated Protein Science (CIPSM), Department Chemie, Technische Universität München, Lichtenbergstr. 4, Garching, D-85747, Germany.

PMID: 25981929
DOI: 10.1002/adma.201500900

Abstract

Orthogonally functionalized binary micropatterned substrates are produced using a novel protocol. The use of adequate peptido-mimetics enables an unprecedented segregation of purified αvβ3 and α5β1 integrins in adjacent microislands and evidences the preference of U2OS cells to colocalize such receptors. Moreover, this tendency can be altered by varying the geometry and composition of the micropatterns.

Keywords: biomimetics; cell adhesion and migration; integrins; micropatterns; orthogonal functionalization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actin Cytoskeleton / metabolism
Biomimetic Materials
Cell Adhesion / physiology
Cell Line, Tumor
Cell Movement / physiology*
Cell Nucleus / metabolism
Culture Media
Ferric Compounds
Focal Adhesions / metabolism*
Gold
Humans
Integrin alpha5beta1 / metabolism*
Integrin alphaVbeta3 / metabolism*
Molecular Structure
Surface Properties
Titanium
Vinculin / metabolism

Substances

Culture Media
Ferric Compounds
Integrin alpha5beta1
Integrin alphaVbeta3
Vinculin
titanium dioxide
ferric oxide
Gold
Titanium