Before there was access to space, all experiments on plant tropisms were conducted upon the background of gravity. The gravity vector could be disrupted, such as with clinorotation and random positioning machines, and by manipulating incident angles of root growth with respect to gravity, such as with Darwin's plants on slanted plates, but gravity could not be removed from the experimental equation. Access to microgravity through spaceflight has opened new doors to plant research. Here we provide an overview of some of the methodologies of conducting plant research in the unique spaceflight environment.