Implantable vaccine development using in vitro antigen-pulsed macrophages absorbed on laser micro-structured Si scaffolds

Vaccine. 2015 Jun 17;33(27):3142-9. doi: 10.1016/j.vaccine.2015.04.017. Epub 2015 May 13.

Abstract

To overcome the limiting antigenic repertoire of protein sub-units and the side effects of adjuvants applied in second generation vaccines, the present work combined in vitro and in vivo manipulations to develop biomaterials allowing natural antigen-loading and presentation in vitro and further activation of the immune response in vivo. 3-dimensional laser micro-textured implantable Si-scaffolds supported mouse macrophage adherence, allowed natural seeding with human serum albumin (antigen) and specific antibody and inflammatory cytokine production in vitro. Implantation of Si-scaffolds loaded with antigen-activated macrophages induced an inflammatory reaction along with antigen-specific antibody production in vivo, which could be detected even 30 days post implantation. Analysis of implant histology using scanning electron microscopy showed that Si-scaffolds could be stable for a 6-month period. Such technology leads to personalized implantable vaccines, opening novel areas of research and treatment.

Keywords: Immune response; Laser manufacturing; Macrophage; Scaffold implantation; Silicon scaffold; Vaccination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens / immunology
  • Antigens / metabolism
  • Cell Adhesion
  • Cell Transplantation*
  • Macrophage Activation
  • Macrophages / immunology*
  • Macrophages / physiology*
  • Male
  • Mice, Inbred BALB C
  • Tissue Scaffolds*
  • Treatment Outcome
  • Vaccination / methods*
  • Vaccines / administration & dosage*

Substances

  • Antigens
  • Vaccines