Molecular PET Imaging of Cyclophosphamide Induced Apoptosis with 18F-ML-8

Biomed Res Int. 2015:2015:317403. doi: 10.1155/2015/317403. Epub 2015 Apr 9.

Abstract

In this paper, a novel small-molecular apoptotic PET imaging probe, (18)F-ML-8 with a malonate motif structure, is presented and discussed. After study, the small tracer that belongs to a member of ApoSense family is proved to be capable of imaging merely apoptotic regions in the CTX treated tumor-bearing mice. The experimental result is further confirmed by in vitro cell binding assays and TUNEL staining assay. As a result, (18)F-ML-8 could be used for noninvasive visualization of apoptosis induced by antitumor chemotherapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • Cell Line, Tumor
  • Chromatography, High Pressure Liquid
  • Cyclophosphamide / pharmacology*
  • Female
  • Humans
  • In Situ Nick-End Labeling
  • Injections, Intravenous
  • Jurkat Cells
  • Methylmalonic Acid / administration & dosage
  • Methylmalonic Acid / analogs & derivatives*
  • Methylmalonic Acid / chemical synthesis
  • Methylmalonic Acid / chemistry
  • Methylmalonic Acid / isolation & purification
  • Methylmalonic Acid / pharmacology*
  • Mice, Nude
  • Molecular Imaging / methods*
  • Positron-Emission Tomography / methods*
  • Tissue Distribution

Substances

  • 2-(3-fluoropropyl)-2-methylmalonic acid
  • Methylmalonic Acid
  • Cyclophosphamide