A procedure for the preparation and isolation of nucleoside-5'-diphosphates

Heidi J Korhonen; Hannah L Bolt; David R W Hodgson

doi:10.3762/bjoc.11.52

A procedure for the preparation and isolation of nucleoside-5'-diphosphates

Beilstein J Org Chem. 2015 Apr 10:11:469-72. doi: 10.3762/bjoc.11.52. eCollection 2015.

Authors

Heidi J Korhonen¹, Hannah L Bolt², David R W Hodgson²

Affiliations

¹ Department of Chemistry, Science Laboratories, Durham University, South Road, Durham, DH1 3LE, United Kingdom, ; Department of Chemistry, University of Turku, Vatselankatu 2, 20014 Turku, Finland.
² Department of Chemistry, Science Laboratories, Durham University, South Road, Durham, DH1 3LE, United Kingdom.

Abstract

Tris[bis(triphenylphosphoranylidene)ammonium] pyrophosphate (PPN pyrophosphate) was used in the SN2 displacements of the tosylate ion from 5'-tosylnucleosides to afford nucleoside-5'-diphosphates. Selective precipitation permitted the direct isolation of nucleoside-5'-diphosphates from crude reaction mixtures.

Keywords: NDP synthesis; nucleic acids; nucleoside-5’-diphosphate; phosphorylation.