Improvements of axial resolution in confocal microscopy with fan-shaped apertures

Appl Opt. 2015 Feb 20;54(6):1354-62. doi: 10.1364/AO.54.001354.

Abstract

Based on diffraction theory, this paper theoretically demonstrates improvements in axial resolution in confocal microscopy with fan-shaped apertures. It provides the optimal geometric parameters of the fan-shaped pupils to give the maximum axial resolution for a given pinhole size. To fully understand the overall performance, the signal-to-background ratio and the signal level with regard to the geometric parameters are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Equipment Design
  • Image Processing, Computer-Assisted / methods
  • Imaging, Three-Dimensional
  • Lasers
  • Microscopy, Confocal / instrumentation*
  • Microscopy, Confocal / methods*
  • Optical Phenomena*
  • Reproducibility of Results
  • Signal-To-Noise Ratio
  • Software