Association of tumor necrosis factor-α promoter G-308A gene polymorphism with increased triglyceride level of subjects with metabolic syndrome

Seyed Reza Mirhafez; Amir Avan; Alireza Pasdar; Elaheh Kazemi; Faezeh Ghasemi; Amir Tajbakhsh; Samaneh Tabaee; Gordon A Ferns; Majid Ghayour-Mobarhan

doi:10.1016/j.gene.2015.05.019

Association of tumor necrosis factor-α promoter G-308A gene polymorphism with increased triglyceride level of subjects with metabolic syndrome

Gene. 2015 Aug 15;568(1):81-4. doi: 10.1016/j.gene.2015.05.019. Epub 2015 May 9.

Authors

Seyed Reza Mirhafez¹, Amir Avan², Alireza Pasdar³, Elaheh Kazemi⁴, Faezeh Ghasemi⁵, Amir Tajbakhsh⁵, Samaneh Tabaee⁶, Gordon A Ferns⁷, Majid Ghayour-Mobarhan⁸

Affiliations

¹ Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran; Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
² Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Biochemistry of Nutrition Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
³ Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Division of Applied Medicine, Medical School, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK.
⁴ Department of Biochemistry, International Campus of Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
⁵ Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
⁶ Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran.
⁷ Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex BN1 9PH, UK.
⁸ Biochemistry of Nutrition Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: ghayourm@mums.ac.ir.

PMID: 25967388
DOI: 10.1016/j.gene.2015.05.019

Abstract

Background: The G-308A single nucleotide polymorphism (SNP) in the promoter of tumor necrosis factor-alpha (TNF-α) gene has previously been reported to be associated with cardiovascular risk. However the potential association of this polymorphism with metabolic syndrome (MetS) is unclear. The aim of the current study was to explore the association of this TNF-α gene polymorphism with MetS in an Iranian population.

Methods/patients: Two hundred and twenty two subjects were recruited and anthropometric and biochemical parameters were determined. Genotyping was performed using the amplification refractory mutation system polymerase chain reaction. The association of the genetic-polymorphism with MetS was evaluated by univariate and multivariate analyses.

Results: MetS subjects had a significantly (P<0.05) higher level of fasted serum triglycerides, body-mass-index, waist-circumference, blood pressure and fasting-blood-glucose, and lower level of high-density lipoprotein cholesterol. The possession of AA or GA genotype of the TNF-α gene was not associated with MetS in our population. However the AA genotype of TNF-α was related to an increased level of triglyceride in MetS patients, compared to the control group.

Conclusion: TNF-α G-308A polymorphism is unlikely to play an important role in the development of MetS in our population.

Keywords: G-308A polymorphism; Metabolic syndrome; Tumor necrosis factor-alpha.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Case-Control Studies
Cross-Sectional Studies
Female
Genetic Association Studies
Genetic Predisposition to Disease
Humans
Male
Metabolic Syndrome / blood
Metabolic Syndrome / genetics*
Middle Aged
Polymorphism, Single Nucleotide
Promoter Regions, Genetic*
Triglycerides / blood*
Tumor Necrosis Factor-alpha / genetics*

Substances

TNF protein, human
Triglycerides
Tumor Necrosis Factor-alpha