Enzyme-Responsive Delivery of Multiple Proteins with Spatiotemporal Control

Adv Mater. 2015 Jun 24;27(24):3620-5. doi: 10.1002/adma.201500417. Epub 2015 May 12.

Abstract

Orchestrated biological materials such as enzymes and growth factors regulate the growth of tissues and organs. A chirality-controlled, single-protein technology is devised to tailor the spatiotemporally defined delivery of therapeutic proteins in response to natural enzymes present at wound sites. Sustained delivery of one protein and sequential delivery of two proteins are demonstrated for stroke and skin wound healing.

Keywords: chiral peptides; enzyme responsive; protein delivery; sequential release; wound healing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Dermis / drug effects
  • Dermis / metabolism
  • Diabetes Mellitus, Experimental / drug therapy
  • Diabetes Mellitus, Experimental / physiopathology
  • Drug Delivery Systems / methods*
  • Endothelial Cells / drug effects
  • Endothelial Cells / metabolism
  • Enzymes / metabolism*
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Humans
  • Mice, Inbred BALB C
  • Mice, Mutant Strains
  • Nanostructures*
  • Proteins / administration & dosage*
  • Proto-Oncogene Proteins c-sis / administration & dosage
  • Skin / drug effects
  • Skin / injuries
  • Stroke / drug therapy
  • Stroke / metabolism
  • Stroke / pathology
  • Vascular Endothelial Growth Factor A / administration & dosage
  • Wound Healing / drug effects

Substances

  • Enzymes
  • Proteins
  • Proto-Oncogene Proteins c-sis
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A