Lentivirus mediated over expression of CGRP inhibited oxidative stress in Schwann cell line

Abstract

CGRP is reported to be implicated in the process of diabetes and neuronal disease. However, the role and underlying mechanism of CGRP involved in diabetic neuropathy is unknown. Schwann cells play a central role in diabetic neuropathy, therefore the protective effect of CGRP on Schwann cells exposed to high glucose is determined. In the present study, full-length CGRP cDNA was isolated and then transferred to gateway adapted lentivirus expression vector by LR recombination reaction. Afterwards, the CGRP bearing recombinant virus was prepared in 293 FT cells and used to infect Schwann cells. The viability and superoxide anions of Schwann cells were evaluated following stimulation with high glucose, and levels of SOD, MDA and NOX1 were assessed. The results suggested that CGRP expression was up-regulated following lentivirus transfection. Lenti-CGRP increased cell viability in high glucose, but the effect was transient. Further lenti-CGRP protected against oxidative stress in Schwann cells triggered by high glucose and lenti-CGRP was effective in increasing SOD and decreasing MDA level. Meanwhile, the increased level of NOX1 caused by high glucose was reversed by lenti-CGRP overexpression. We therefore, suggest that lenti-CGRP may play a role in inhibiting oxidative stress in Schwann cell lines following hyperglycemic stimulation.

Keywords: CGRP; Oxidative stress; Schwann cell.