[Analysis on 2011 quality control results on aerobic plate count of microbiology laboratories in China]

Wei Sheng Yan Jiu. 2015 Jan;44(1):73-6.
[Article in Chinese]

Abstract

Objective: To test the aerobic plate count examining capability of microbiology laboratories, to ensure the accuracy and comparability of quantitative bacteria examination results, and to improve the quality of monitoring.

Methods: The 4 different concentration aerobic plate count piece samples were prepared and noted as I, II, III and IV. After homogeneity and stability tests, the samples were delivered to monitoring institutions. The results of I, II, III samples were logarithmic transformed, and evaluated with Z-score method using the robust average and standard deviation. The results of IV samples were evaluated as "satisfactory" when reported as < 10 CFU/piece or as "not satisfactory" otherwise. Pearson χ2 test was used to analyze the ratio results.

Results: 309 monitoring institutions, which was 99.04% of the total number, reported their results. 271 institutions reported a satisfactory result, and the satisfactory rate was 87.70%. There was no statistical difference in satisfactory rates of I, II and III samples which were 81.52%, 88.30% and 91.40% respectively. The satisfactory rate of IV samples was 93.33%. There was no statistical difference in satisfactory rates between provincial and municipal CDC.

Conclusion: The quality control program has provided scientific data that the aerobic plate count capability of the laboratories meets the requirements of monitoring tasks.

MeSH terms

  • Bacteria / growth & development
  • Bacteria / isolation & purification*
  • China
  • Clinical Laboratory Techniques / standards*
  • Colony Count, Microbial / methods*
  • Colony Count, Microbial / standards
  • Laboratories / standards
  • Quality Control*