Magnetic resonance imaging of tumor with a self-traceable polymer conjugated with an antibody fragment

Hisatsugu Yamada; Yoshinori Hasegawa; Yuki Suzuki; Hirohiko Imai; Tetsuya Matsuda; Yu Kimura; Akio Toshimitsu; Yasuhiro Aoyama; Teruyuki Kondo

doi:10.1016/j.bmcl.2015.04.072

Magnetic resonance imaging of tumor with a self-traceable polymer conjugated with an antibody fragment

Bioorg Med Chem Lett. 2015 Jul 1;25(13):2675-8. doi: 10.1016/j.bmcl.2015.04.072. Epub 2015 Apr 30.

Authors

Hisatsugu Yamada¹, Yoshinori Hasegawa², Yuki Suzuki², Hirohiko Imai³, Tetsuya Matsuda³, Yu Kimura⁴, Akio Toshimitsu⁵, Yasuhiro Aoyama⁶, Teruyuki Kondo⁷

Affiliations

¹ Advanced Biomedical Engineering Research Unit, Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
² Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
³ Department of Systems Science, Graduate School of Informatics, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan.
⁴ Research and Educational Unit of Leaders for Integrated Medical System, Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan.
⁵ Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan; Division of Multidisciplinary Chemistry, Institute for Chemical Research, Kyoto University, Gokanosho, Uji, Kyoto 611-0011, Japan.
⁶ Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. Electronic address: aoyama.yasuhiro.78z@st.kyoto-u.ac.jp.
⁷ Advanced Biomedical Engineering Research Unit, Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan; Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. Electronic address: teruyuki@scl.kyoto-u.ac.jp.

PMID: 25958246
DOI: 10.1016/j.bmcl.2015.04.072

Abstract

A (13)C-enriched phosphorylcholine polymer ((13)C-PMPC) as a self-traceable MR (magnetic resonance) tag was conjugated with a fragment (scFv) of Herceptin, a clinical antibody against antigen Her2. When injected in model mice bearing Her2(+) (gastric) and Her2(-) (pancreatic) tumors, the antibody-tag conjugate (13)C-PMPC-scFv selectively accumulated in the Her2(+) tumor with a rapid build-up/decay (accumulation/clearance) profile and, with the use of the (1)H-(13)C double-resonance (heteronuclear correlation) technique, the Her2(+) gastric tumor was clearly MR imaged.

Keywords: Active targeting; Anti-Her2 antibody; MRI; Self-traceable polymer; Tumor imaging.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carbon Isotopes / pharmacokinetics
Cell Line, Tumor
Female
Humans
Immunoconjugates / chemistry
Immunoconjugates / pharmacokinetics*
Magnetic Resonance Imaging
Mice
Mice, Inbred BALB C
Mice, Nude
Pancreatic Neoplasms / diagnosis*
Pancreatic Neoplasms / metabolism
Phosphorylcholine / analogs & derivatives
Phosphorylcholine / pharmacokinetics
Polymethacrylic Acids / pharmacokinetics
Receptor, ErbB-2 / immunology
Receptor, ErbB-2 / metabolism
Single-Chain Antibodies / pharmacokinetics
Stomach Neoplasms / diagnosis*
Stomach Neoplasms / metabolism

Substances

Carbon Isotopes
Immunoconjugates
Polymethacrylic Acids
Single-Chain Antibodies
poly(2-methacryloyloxyethyl-phosphorylcholine)
Phosphorylcholine
ERBB2 protein, human
Receptor, ErbB-2