Graphene-CNT nanohybrid aptasensor for label free detection of cardiac biomarker myoglobin

Biosens Bioelectron. 2015 Oct 15:72:56-60. doi: 10.1016/j.bios.2015.04.089. Epub 2015 Apr 28.

Abstract

We report a label free electrochemical detection of cardiac bio-marker myoglobin (Mb) on aptamer functionalized rGO/CNT nanostructured electrodes by measuring its direct electron transfer (DET). Configured as a highly responsive aptasensor, the newly developed biosensing platform exhibits synergistic effect of the nano-hybrid functional construct by combining good electrical properties and the facile chemical functionality of nanohybrid for the compatible bio-interface development. The specific anti-Mb aptamer was generated by five iterative SELEX (Systematic evolution of ligands by exponential enrichment) rounds, showing high senstivity (KD ~65 pM). The aptamer functionalized rGO/CNT nanostructured electrodes demonstrated a significant increase in signal response with a detection limit of ~0.34 ng/mL in the dynamic response range between 1 ng/mL and 4 µg/mL for Mb. The newly developed DET assay format presents a promising candidate in point-of-care diagnosis for routine screening of Mb in patient's samples.

Keywords: Aptamer; Cardiac marker; Direct electron transfer; Myoglobin; rGO/CNT nanohybrid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Biomarkers / analysis
  • Biosensing Techniques / instrumentation
  • Electrochemical Techniques / instrumentation*
  • Electrodes
  • Graphite / chemistry*
  • Humans
  • Limit of Detection
  • Models, Molecular
  • Myoglobin / analysis*
  • Nanotubes, Carbon / chemistry*
  • Nanotubes, Carbon / ultrastructure
  • Point-of-Care Systems
  • SELEX Aptamer Technique

Substances

  • Aptamers, Nucleotide
  • Biomarkers
  • Myoglobin
  • Nanotubes, Carbon
  • Graphite