Up-and-down-shaker-assisted dispersive liquid-liquid microextraction coupled with gas chromatography-mass spectrometry for the determination of fungicides in wine

Shang-Ping Chu; Wan-Chi Tseng; Po-Hsin Kong; Chun-Kai Huang; Jung-Hsuan Chen; Pai-Shan Chen; Shang-Da Huang

doi:10.1016/j.foodchem.2015.04.015

Up-and-down-shaker-assisted dispersive liquid-liquid microextraction coupled with gas chromatography-mass spectrometry for the determination of fungicides in wine

Food Chem. 2015 Oct 15:185:377-82. doi: 10.1016/j.foodchem.2015.04.015. Epub 2015 Apr 11.

Authors

Shang-Ping Chu¹, Wan-Chi Tseng¹, Po-Hsin Kong², Chun-Kai Huang³, Jung-Hsuan Chen⁴, Pai-Shan Chen⁵, Shang-Da Huang⁶

Affiliations

¹ Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
² Department and Graduate Institute of Forensic Medicine, National Taiwan University, Taipei 10002, Taiwan.
³ Department of Chemistry, National Chung-Hsing University, Taichung 40227, Taiwan.
⁴ Department and Graduate Institute of Forensic Medicine, National Taiwan University, Taipei 10002, Taiwan; Forensic and Clinical Toxicology Center, National Taiwan University College of Medicine and National Taiwan University Hospital, Taipei 10002, Taiwan.
⁵ Department and Graduate Institute of Forensic Medicine, National Taiwan University, Taipei 10002, Taiwan; Forensic and Clinical Toxicology Center, National Taiwan University College of Medicine and National Taiwan University Hospital, Taipei 10002, Taiwan. Electronic address: paishanchen@ntu.edu.tw.
⁶ Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan. Electronic address: sdhuang@mx.nthu.edu.tw.

PMID: 25952882
DOI: 10.1016/j.foodchem.2015.04.015

Abstract

An up-and-down-shaker-assisted dispersive liquid-liquid microextraction (UDSA-DLLME) method coupled with gas chromatography-mass spectrometry was developed for the determination of fungicides (cyprodinil, procymidone, fludioxonil, flusilazole, benalaxyl, and tebuconazole) in wine. The developed method requires 11 μL of 1-octanol without the need for dispersive solvents. The total extraction time was approximately 3 min. Under optimum conditions, the linear range of the method was 0.05-100 μg L(-1) for all fungicides and the limit of detection was 0.007-0.025 μg L(-1). The absolute and relative recoveries were 31-83% and 83-107% for white wine, respectively, and 32-85% and 83-108% for red wine, respectively. The intra-day and inter-day precision were 0.5-7.5% and 0.7-6.1%, respectively. Our developed method had good sensitivity and high extraction efficiency. UDSA-DLLME is a desirable method in terms of performance and speed.

Keywords: Fungicides; Gas chromatography; Improved solvent collection system; Up-and-down-shaker-assisted dispersive liquid–liquid microextraction (UDSA–DLLME); Wine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fungicides, Industrial / analysis*
Gas Chromatography-Mass Spectrometry / methods*
Limit of Detection
Liquid Phase Microextraction / methods*
Wine / analysis*

Substances

Fungicides, Industrial