Composition and microstructure of colostrum and mature bovine milk fat globule membrane

Xiaoqiang Zou; Zheng Guo; Qingzhe Jin; Jianhua Huang; Lingzhi Cheong; Xuebing Xu; Xingguo Wang

doi:10.1016/j.foodchem.2015.03.145

Composition and microstructure of colostrum and mature bovine milk fat globule membrane

Food Chem. 2015 Oct 15:185:362-70. doi: 10.1016/j.foodchem.2015.03.145. Epub 2015 Apr 4.

Authors

Xiaoqiang Zou¹, Zheng Guo², Qingzhe Jin³, Jianhua Huang⁴, Lingzhi Cheong⁵, Xuebing Xu⁶, Xingguo Wang⁷

Affiliations

¹ State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, PR China; Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark. Electronic address: xiaoqiangzou@163.com.
² Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark. Electronic address: guo@mb.au.dk.
³ State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, PR China. Electronic address: jqzwuxi@163.com.
⁴ State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, PR China. Electronic address: huangjianhua1124@sina.com.cn.
⁵ Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark. Electronic address: lingzhicheong@yahoo.com.
⁶ Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark. Electronic address: xu@mb.au.dk.
⁷ State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, PR China. Electronic address: wxg1002@qq.com.

PMID: 25952880
DOI: 10.1016/j.foodchem.2015.03.145

Abstract

The microstructures of colostrum and mature bovine milk fat globule membrane (MFGM) were investigated using confocal laser scanning microscopy (CLSM) at different temperatures, and the relationships between microstructure variations and the chemical compositions of the MFGM were also examined. Using a fluorophore-labeled phospholipid probe, we found that non-fluorescent domains on the MFGM were positively correlated with the amount of sphingomyelin at both room (20 °C) and physiological (37 °C) temperatures. However, at the storage temperature (4 °C), there were more non-fluorescent domains on the MFGM. These results indicate that the heterogeneities in the MFGM are most likely to be the result of the lateral segregation of sphingomyelin at the room and physiological temperatures, and at the storage temperature, phospholipids with saturated fatty acids affect the formation of these domains.

Keywords: Confocal laser scanning microscopy; Microstructure variations; Milk fat globule membrane; Phospholipids; Sphingomyelin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Colostrum / chemistry*
Fatty Acids / analysis
Female
Glycolipids / analysis
Glycoproteins / analysis
Glycoproteins / ultrastructure*
Lipid Droplets
Microscopy, Confocal / methods
Sphingomyelins / analysis

Substances

Fatty Acids
Glycolipids
Glycoproteins
Sphingomyelins
milk fat globule