A novel ultrasensitive electrochemical DNA sensor based on double tetrahedral nanostructures

Biosens Bioelectron. 2015 Sep 15:71:434-438. doi: 10.1016/j.bios.2015.04.065. Epub 2015 Apr 23.

Abstract

Electrochemical DNA (E-DNA) sensor is an important tool for detecting DNA biomarker. In this work, we have demonstrated a novel strategy of E-DNA sensor based on DNA tetrahedral nanostructures for the sensitive detection of target DNA. In our design, thiol and biotin modified DNA tetrahedral nanostructures were used as capture and report probes respectively. The biotin-tagged three dimensional DNA tetrahedral nanostructures were employed for efficient signal amplification by capturing multiple catalytic enzymes. Such improved E-DNA sensor can sensitively detect DNA target as low as 1 fM with excellent differentiation ability for even single mismatch. And a mean recovery rate of 90.57% in DNA solution extracted from human serum was obtained. We have also compared this new method of attaching catalytic enzymes with the other two typical methods: One is through biotinylated single-stranded DNA (SSDNA) and the other is through gold nanoparticles (GNPs). Results indicated that the RTSPs-based enzyme amplification system showed much better performance than the other two systems.

Keywords: DNA tetrahedral nanostructure; Electrochemical DNA sensors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Conductometry / instrumentation*
  • DNA / analysis*
  • DNA / chemistry
  • DNA / genetics*
  • DNA Probes / chemistry
  • DNA Probes / genetics*
  • Equipment Design
  • Equipment Failure Analysis
  • Oligonucleotide Array Sequence Analysis / instrumentation*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • DNA Probes
  • DNA