Microfluidic impedance flow cytometry enabling high-throughput single-cell electrical property characterization

Jian Chen; Chengcheng Xue; Yang Zhao; Deyong Chen; Min-Hsien Wu; Junbo Wang

doi:10.3390/ijms16059804

Microfluidic impedance flow cytometry enabling high-throughput single-cell electrical property characterization

Int J Mol Sci. 2015 Apr 29;16(5):9804-30. doi: 10.3390/ijms16059804.

Authors

Jian Chen¹, Chengcheng Xue², Yang Zhao³, Deyong Chen⁴, Min-Hsien Wu⁵, Junbo Wang⁶

Affiliations

¹ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. chenjian@mail.ie.ac.cn.
² State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. xuechengcheng13@mails.ucas.ac.cn.
³ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. zhaoyang110@mails.ucas.ac.cn.
⁴ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. dychen@mail.ie.ac.cn.
⁵ Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan 333, Taiwan. mhwu@mail.cgu.edu.tw.
⁶ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. jbwang@mail.ie.ac.cn.

Abstract

This article reviews recent developments in microfluidic impedance flow cytometry for high-throughput electrical property characterization of single cells. Four major perspectives of microfluidic impedance flow cytometry for single-cell characterization are included in this review: (1) early developments of microfluidic impedance flow cytometry for single-cell electrical property characterization; (2) microfluidic impedance flow cytometry with enhanced sensitivity; (3) microfluidic impedance and optical flow cytometry for single-cell analysis and (4) integrated point of care system based on microfluidic impedance flow cytometry. We examine the advantages and limitations of each technique and discuss future research opportunities from the perspectives of both technical innovation and clinical applications.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Electric Impedance
Flow Cytometry / methods*
High-Throughput Screening Assays / methods*
Humans
Microfluidics / methods*
Point-of-Care Systems
Single-Cell Analysis / methods*