In the north of the Pskov region (58 degrees 35' N, 28 degrees 55' E) the appearance of a single colony of true bugs Pyrrhocoris apterus has been recorded. Dissection of 95 individuals from this colony revealed 100% prevalence of infection with homoxenous trypanosomatids. In 3% of the cases intestinal infection was accompanied by hyperinvasion into the salivary glands and hemolyph of the hosts. Analysis of trypanosomatid morphotypes demonstrated mixed infections in all studied P. apterus individuals. At least 4 forms of promastigotes along with epimastigotes, choanomastigotes and amastigotes were found. The distribution of the trypanosomatid morphotypes over all intestinal parts as well as salivary glands and hemolymph was investigated. Three isolates of the flagellates were deposited into the living cultures collection of the laboratory of Protozoology of the Zoological institute of the Russian Academy of Sciences.